DETEKSI MUTASI V1016G PADA GEN VOLTAGE-GATED SODIUM CHANNEL PADA POPULASI Aedes aegypti (DIPTERA: CULICIDAE) DI KABUPATEN KLATEN, JAWA TENGAH DENGAN METODE ALLELE-SPECIFIC PCR

Dyah Widiastuti, Sunaryo Sunaryo, Nova Pramestuti, Tika Fiona Sari, Nastiti Wijayanti

Abstract


Abstrak

Meluasnya kejadian resistensi pada vektor virus Dengue di Jawa Tengah memerlukan strategi pengelolaan resistensi insektisida secara efektif. Oleh karena itu, informasi mengenai mutasi gen pada posisi 1016 di domain II segmen ke­6 gen VGSC pada nyamuk Aedes aegypti yang menyebabkan perubahan asam amino valin (V) menjadi glisin (G) akan dapat memperkuat penelitian operasional mengenai strategi pemilihan insektisida dalam program­pengendalian­vektor­Dengue.­Penelitian­ini­menggunakan­uji­Allele-Specific­Polymerase­Chain­Reaction(AS­PCR) yang dapat mendeteksi mutasi V1016G. Sampel penelitian ini adalah 22 ekor nyamuk Aedes aegypti dari Kabupaten Klaten yang berumur 2­5 hari. Hasil penelitian menunjukkan bahwa 22,7% nyamuk belum mengalami mutasi (V/V), 59,1% nyamuk mengalami mutasi heterozigot (V/G) dan 18,2% nyamuk mengalami mutasi homozigot (G/G). Hal ini menunjukkan indikasi terjadinya resistensi populasi nyamuk Ae.aegypti terhadap insektisida sintetik piretroid yang disebabkan oleh mekanisme knockdown resistance.

Kata Kunci:­Aedes­aegypti,­mutasi­V1016G,­Allele-Specific­PCR,­VGSC

Abstract

Insecticides resistance has spread rapidly among dengue vectors from Central Java, and require an effective insecticide resistance management strategies.one of the resistance mechanism in Aedes aegypti may arise through knockdown resistance or kdr which consists of single point mutation within the genes that are targeted by insecticide compounds. Mutation at position 1016 in domain II, segment 6 of the Voltage Gated Sodium Channel gene in Ae. aegypti leads to a valine to glycine substitution (V1016G) is associated with resistance to the type II pyrethroid. The result of this study will help us to strengthen basic and operational research on the­development­of­strategies­for­Dengue­vector­control­in­Indonesia.­This­study­utilized­an­allele-specificPolymerase Chain Reaction (AS­PCR) assay that could be used to detect the V1016G mutation. The assay was conducted on 22 female mosquitoes aged 2–5 days old. The result showed there were 22,7% wild type mosquito (V/V), 59,1% heterozygous for V1016G mutation (V/G) and 18,2% V1016G mutant homozygous (G/G). It indicated synthetic pyrethroid resistance in Ae.aegypti population caused by knockdown resistance mechanism.

Keywords: Aedes aegypti,­V1016G­mutation,­Allele-Specific­PCR,­VGSC


Keywords


Aedes aegypti,­V1016G­mutation,­Allele-Specific­PCR,­VGSC

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p-ISSN : 2085-868X

e-ISSN : 2354-8789

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Published by Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan Republik Indonesia

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