EFIKASI Bacillus thuringiensis H-14 ISOLAT SALATIGA SEDIAAN BUBUK DAN CAIR TERHADAP JENTIK Culex quinquefasciatus

Yusnita M. Anggraeni, Esti Rahardianingtyas, Rendro Wianto

Abstract


Abstrak

Filariasis merupakan salah satu penyakit tular vektor yang masih menjadi masalah di Indonesia dengan prevalen­si­19%­pada­tahun­2009.­Salah­satu­nyamuk­yang­menjadi­vektor­filariasis­adalah­Culex­quinquefasciatus.Pengendalian nyamuk vektor dengan Bacillus thuringiensis H­14 (Bt H­14) isolat Salatiga sediaan cair dan bubuk­merupakan­salah­satu­cara­mengendalikan­filariasis­secara­hayati.­Penelitian­ini­bertujuan­untukmenentukan­efikasi­Bt­H-14­isolat­Salatiga­sediaan­cair­dan­bubuk­terhadap­Cx­quinquefasciatus.­Penelitiandilakukan dengan membuat Bt H­14 isolat Salatiga sediaan cair dan bubuk, menghitung jumlah sel dan spora pada masing­masing sediaan serta mengujikan pada jentik Cx. quinquefasciatus hasil pemeliharaan insektarium B2P2VRP Salatiga. Nilai kematian kemudian dianalisis menggunakan analisis Probit Hasil penelitian menunjukkan sediaan cair memiliki jumlah sel 1,55x108 sel/ml sedangkan sediaan bubuk 1,72x106 sel/g. Nilai LC90 Bt H­14 sediaan cair dan bubuk terhadap Cx. quinquefasciatus berturut­turut 0,056 ppm dan 10,94 ppm. Hal ini menunjukkan potensi keduanya dalam membunuh jentik Cx. quinquefasciatus sebagai vektor­filariasis.

Kata kunci:­Bacillus­thuringiensis,­cair,­bubuk,­efikasi,­Culex­quinquefasciatus,

Abstract

Filariasis as a vector­borne disease become a problem in Indonesia with prevalence of 19% in 2009. Culex quinquefasciatus is one of its vectors. Larvae control using Salatiga isolate of Bacillus thuringiensis H­14 liquid and­powder­preparations­are­filariasis­mosquito­control­method.­This­study­aimed­to­determine­the­efficacyof Bt H­14 isolates Salatiga liquid and powder preparations against Cx. quinquefasciatus. The study was conducted by formulating Salatiga isolate Bt H­14 liquid and powder preparation, cells and spores counting of Bt H­14 in each preparation, and conducting the toxicity assay on Cx. quinquefasciatus larvae. The mortality was analyzed using Probit analysis. The results showed that liquid preparations had 1,55x108 cells/ml while the powder preparation had 1,72x106 cells/g. LC90 value of Bt H­14 liquid and powder preparations against Cx. quinquefasciatus were respectively 0.056 ppm and 10.94 ppm. This shows the potential of both preparations in killing Cx. quinquefasciatus­larvae­as­vectors­of­filariasis.

Keywords: Bacillus thuringiensis,­liquid,­powder,­efficacy,­Culex quinquefasciatus


Keywords


Bacillus thuringiensis,­liquid,­powder,­efficacy,­Culex quinquefasciatus

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p-ISSN : 2085-868X

e-ISSN : 2354-8789

LIPI Accreditation Number : 583/Akred/P2MI-LIPI/09/2014

Published by Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan Republik Indonesia

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