Detection of Markers of Increased Virulence Non Structural protein (NS I) Avian Influenza Virus H5N1 from Indonesia=DETEKSI PENANDA PENINGKATAN VIRULENSI NON STRUKTURAL PROTEIN (NS1) VIRUS AVIAN INFLUENZA H5N1 ASAL INDONESIA

Arief Mulyono

Detection of Markers of Increased Virulence Non Structural protein (NS I) Avian Influenza Virus H5N1 from Indonesia=DETEKSI PENANDA PENINGKATAN VIRULENSI NON STRUKTURAL PROTEIN (NS1) VIRUS AVIAN INFLUENZA H5N1 ASAL INDONESIA

Abstract


ENGLISH

Abstract

NS1 protein is a multifunction protein that plays key role of pathogenesis and virulence of avians influenza virus H5N1. The amino acid substitution at the position P42S, D92E, F103I, M106I and 5 amino acid deletion at the position 80 to 84 in NS1 protein reported increasing virulence of avians influenza virus H5N1. Several studies showed avians influenza virus H5N1 in Indonesia has dynamic changed. This study aimed to analyze the markers of virulence of NS1 protein sequences of all H5N1 virus isolates from Indonesia. The source of NS1 protein sequence data gene obtained from GeneBank and Gisaid. Data were analyzed using Bioedit software. The Results showed the isolates from Indonesia had substitutions P42S and 5 amino acids deletions at positions 80-84 resulting in the potential for increased virulence of the virus. However, amino acid substitution at the position D92E, F103L and M106I substitution were not found.

INDONESIAN

Abstrak
Protein NS1 adalah protein multifungsi yang memainkan peran kunci dalam patogenesis dan virulensi virus avian influenza H5N1. Substitusi asam amino P42S, D92E, F103I, M106I, dan delesi 5 asam amino di posisi 80 - 84 dilaporkan meningkatkan virulensi virus avian influenza H5N1. Beberapa penelitian menunjukkan bahwa virus avian influenza di Indonesia mengalami perubahan dinamis. Studi ini akan menganalisis motif asam amino yang menjadi penanda peningkatan virulensi pada sekuen protein NS1 virus avian influenza H5N1 asal Indonesia. Data sekuen asam amino protein NS1 diperoleh dari database GeneBank dan Gisaid. Analisis data menggunakan Bioedit software. Hasil analisis menunjukkan subtitusi asam amino dari prolin ke serin di posisi 42 (P42S) dan delesi 5 asam amino di posisi 80 – 84 telah ditemukan pada virus avian influenza asal Indonesia, akan tetapi tidak ditemukan substitusi asam amino aspartat ke glutamat diposisi no 92 (D92E) dan tidak ada yang mengalami 2 substitusi asam amino sekaligus diposisi 103 dan 106.


Keywords


H5N1, NS1, Indonesia

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Jurnal Kefarmasian Indonesia is a scientific journal published by the Center for Research and Development of Biomedical and Basic Health Technology, Board of Health Research and Development, Ministry of Health of the Republic of Indonesia. The journal publishes original research articles in pharmaceutical science such as Pharmaceutical Technology, Pharmacology, Pharmaceutical Chemistry, Traditional Medicines, and Pharmaceutical Care.

The journal was established in 2009 while online publication has been started from 2015. The journal is published in Bahasa Indonesia and English.  Jurnal Kefarmasian Indonesia is biannual, open access, peer-reviewed, and online pharmacy journal. Jurnal Kefarmasian Indonesia aims to serve the updated scientific knowledge for researchers in pharmaceutical fields. There is no charge for submitted manuscript as well as for processing manuscript. The journal has been registered with e-ISSN 2354-8770, and p-ISSN 2085-675X and accredited by Indonesian Institute of Sciences (LIPI) with Decree No. 581/Akred/P2MI/LIPI/09/2014.

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