ISOLATION OF Bacilllus thuringiensis FROM DIFFERENT HABITS IN THE DISTRICT MAGELANG AND MAGELANG MULTICIPALITY AND PATHOGENICITY AGAINST Aedes aegypti LARVAE=ISOLASI Bacillus thuringiensis DARI BERBAGAI HABITAT DI KABUPATEN DAN KOTA MAGELANG

Esti Rahardianingtyas, Rendro Wianto

ISOLATION OF Bacilllus thuringiensis FROM DIFFERENT HABITS IN THE DISTRICT MAGELANG AND MAGELANG MULTICIPALITY AND PATHOGENICITY AGAINST Aedes aegypti LARVAE=ISOLASI Bacillus thuringiensis DARI BERBAGAI HABITAT DI KABUPATEN DAN KOTA MAGELANG

Abstract


ENGLISH

Dengue hemorrhagic fever (DHF) is still a health problem in Indonesia, transmited by Aedes aegypti. One of vector control method against Aedes aegypti larvae has conducted using Bacillus thuringiensis due to safe for the environment. The objectives of this study was to isolate and identify B. thuringiensis which toxic to Ae. aegypti larvae at various habitat and ecosystem in the District Magelangand magelang multicipality. Twenty-six soil samples collected from 10 locations were isolated and identified in the microbiology laboratory, Institute for Vector and Reservoir Control Research and Development, Central Salatiga. Twenty-six soil samples and 40 isolates were identified positive of B thuringiensis, 29 isolates had ˃50% toxicity and 11 isolates had ˂50% toxicity. Isolate with 90% toxicity were isolated from resting place ecosystem.Serological research needs to be conducted to identify the pathogenicity of isolates with ˃ 50% toxicity against Ae. aegypti larvae.

INDONESIAN

Demam berdarah dengue (DBD) masih merupakan masalah kesehatan di Indonesia. Salah satu upaya pengendalian vektor penyakit tersebut adalah pengendalian terhadap jentik Aedes aegypti menggunakan Bacillus thuringiensis yang efektif dan aman bagi lingkungan. Tujuan penelitian adalah untuk mengisolasi dan mengidentifikasi B. thuringiensis yang toksik terhadap jentik nyamuk Ae. aegypti di berbagai lokasi di Kabupaten dan Kota Magelang. Dua puluh enam sampel tanah diambil dari 10 lokasi diisolasi dan diidentifikasi.di laboratorium mikrobiologi, B2P2VRP Salatiga diperoleh 40 isolat B thuringiensis, dimana 29 isolat yang didapat memiliki toksisitas ˃50% terhadap jentik nyamuk Ae. aegypti sedangkan sebelas isolat yang didapat memiliki toksisitas ˂50% terhadap jentik nyamuk Ae. aegypti. Isolat dengan toksisitas 90% diisolasi dari sampel tanah yang diambil dari habitat makam. Perlu dilakukan penelitian uji serologi dari isolat yang patogenisitasnya ˃50%.


Keywords


Habitat, Isolation, B. thuringiensis, Ae.aegypti, Habitat, Isolasi

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Published by Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga

Jl. Hasanudin No.123 Salatiga

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p-ISSN: 2085-675X

e-ISSN: 2354-8770

Jurnal Kefarmasian Indonesia is a scientific journal published by the Center for Research and Development of Biomedical and Basic Health Technology, Board of Health Research and Development, Ministry of Health of the Republic of Indonesia. The journal publishes original research articles in pharmaceutical science such as Pharmaceutical Technology, Pharmacology, Pharmaceutical Chemistry, Traditional Medicines, and Pharmaceutical Care.

The journal was established in 2009 while online publication has been started from 2015. The journal is published in Bahasa Indonesia and English.  Jurnal Kefarmasian Indonesia is biannual, open access, peer-reviewed, and online pharmacy journal. Jurnal Kefarmasian Indonesia aims to serve the updated scientific knowledge for researchers in pharmaceutical fields. There is no charge for submitted manuscript as well as for processing manuscript. The journal has been registered with e-ISSN 2354-8770, and p-ISSN 2085-675X and accredited by Indonesian Institute of Sciences (LIPI) with Decree No. 581/Akred/P2MI/LIPI/09/2014.

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EISSN: 2354 - 8770

Published by Center for Research and Development of Biomedical and Basic Health Technology

Jl. Percetakan Negara No. 23 Jakarta Pusat 10560

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