Aplikasi Teknik Diagnosis Schistosomiasis Berbasis Molekuler

Anis Nurwidayati


Schistosomiasis ranks second only to malaria in a public health problem in the world.Schistosomiasis in Indonesia is endemic in Central Sulawesi, which is in Lindu, Napu andBada Highland. The disease is caused by trematodes worm, Schistosoma spp and requiresfreshwater snails as the intermediate host. The World Health Organization (WHO)recommends schistosomiasis research focus is the development and evaluation of newstrategies and tools for disease control. This paper aims to describe several moleculartechniques to detect schistosomiasis in human or intermediate snails. This paperarranged by compiled based search journals and scientific articles related moleculartechniques for detecting schistosomiasis using PCR and LAMP. Molecular basedtechniques for schistosomiasis diagnostic have been developed in several countries, suchas pcr and lamp. Several studies have compared the techniques of schistosomiasisdiagnosis in human by polymerase chain reaction (PCR) with the technique of loopmediatedisothermal amplification (LAMP). Several studies show the results of pcr andlamp applications to detect dna schistosoma on snails. Based on the results of variousstudies, it is known applications, advantages and disadvantages of each - each technique.


Schistosomiasis menempati urutan kedua setelah malaria dalam masalah kesehatanmasyarakat di dunia. Schistosomiasis di Indonesia ditemukan endemis di SulawesiTengah, yaitu di Dataran Tinggi Lindu, Napu dan Bada. Penyakit ini disebabkan olehcacing Trematoda, Schistosoma spp dan membutuhkan keong air tawar sebagai hospesperantaranya. Badan Kesehatan Dunia (WHO) merekomendasikan fokus penelitianschistosomiasis adalah pengembangan dan evaluasi strategi dan alat baru untukpengendalian penyakit. Tulisan ini bertujuan untuk mendeskripsikan beberapa teknikmolekuler untuk mendeteksi schistosomiasis baik pada manusia maupun keongperantaranya. Tulisan disusun berdasarkan penelusuran jurnal dan artikel ilmiahterkait teknik molekuler PCR dan LAMP untuk mendeteksi schistosomiasis. Teknikdiagnosis schistosomiasis berdasarkan molekuler telah banyak dikembangkan dibeberapa negara, diantaranya adalah PCR dan LAMP. Beberapa penelitianmembandingkan teknik diagnosis schistosomiasis dengan Polymerase Chain Reaction(PCR) dengan teknik Loop-Mediated Isothermal Amplification (LAMP). Beberapa studimenunjukkan hasil dari aplikasi teknik PCR dan LAMP untuk mendeteksi DNASchistosoma pada keong. Berdasarkan hasil dari berbagai penelitian tersebut, dapatdiketahui aplikasi, kelebihan dan kekurangan dari masing – masing teknik.


Schistosomiasis, DNA, PCR, LAMP, Snails


WHO. Schistosomiasis Fact Sheet. (2010). http://www.who.int; disitasi 11 Oktober 2010; 20.00.

Pinardi, Hadidjaja, Schistosomiasis di Sulawesi Tengah, Indonesia. Jakarta. Fakultas Kedokteran Universitas Indonesia. (1985)

hal: 12-12.

Dinas Kesehatan Propinsi Sulawesi Tengah. Prevalensi Schistosomiasis di Sulawesi Tengah. (2012).

Abath, F.G., A.L. Gomes, F.L. Melo, C.S. Barbosa and R.P. Werkhuser. Molecular Approaches for the detection of Schistosoma mansoni: Possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection. Mem. Inst. Oswaldo Cruz. 2006 (101): 145-148. diakses pada: 20 Maret 2015.

Notomi T, Okayama H, Masubuchi, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-Mediated Isothermal amplification of DNA. Nucleic Acids Res. 2000. 28 (12): E63. doi:10.1093/nar/28.12.e63.PMC 102748. PMID 10871386. diakses pada: 20 Maret 2015.

Da Silva D, S. Sobral-Hamaguchi, R.G.M. Spada, A.Z. Abdel Hamid and N.R.B. Zuim et al., Biomphalaria tenagophila: Genetic variability between intermediate snail hosts susceptible and resistant to Schistosoma mansoni infection. Parasite. 2004 (11): 35-42. diakses pada: 20 Maret 2015.

Lotfy, W.M., R.J. DeJong, B.S. Black and E.S. Loker. Spesific identification of Egyptian Biomphalaria species and possible hybrids using the polymerase chain reaction r and mitochondrbased on nuclear and

mitochondrial loci. Mol. Cell Prob. 2005 (19): 21-25. diakses pada: 20 Maret 2015.

Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell Probes. 2002. 16 (3): 223-229. doi: 10.1006/mcpr.2002.0415. PMID 12144774. diakses pada: 20 Maret 2015.

Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biphys. Res. Commun. 2001. 289 (1): 150-154. doi:10.1006/bbrc.2001.5921. PMID 11708792. diakses pada: 20 Maret 2015.

Mori Y, Kitao M, Tomita N, Notomi T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J. Biochem. Biophys. Methods. 2004 . 59 ( 2 ) : 145 - 157 . doi:10.1016/j.jbbm.2003.12.005. PMID 15163526. diakses pada: 20 Maret 2015.

Tomita N, Mori Y, Kanda H, Notomi T. Loopmediated isothemal amplification (LAMP) of gene sequences and simple visual detection of

products. Nat. Protoc. 2008. 3 (5): 877-882. doi:10.1038/nprot.2008.57. PMID 18451795. diakses pada: 20 Maret 2015.

Parida M, Santosh S, Dash P.K, Rao P.V.L, Morita K. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev. Med. Virol. 2008 ; 18 : 407 - 421.

www.interscience.wiley.com. doi:10.1002/rmv.593 diakses pada: 20 Maret

Curtis KA, Rudolph DL, Owen SM. Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RTLAMP). J. Virol. Methods. 2008. 151 (2): 264-270.doi: 10.1016.j.jviromet.2008.04.011. PMID 18524393. diakses pada: 20 Maret 2015.

Torres C, Vitalis E A, Baker BR, Gardner SN, et al. LAVA: an open-source approach to designing LAMP (loop-mediated isothermal amplification) DNA signatures. 2011. BMC Bioinformatics. 2011. 12 : 240. doi:10.1186/1471-2105-12-240. PMC 3213686. PMID 21679460.

Wang C, Chen L, Yin X, Hua W, Hou M, et al. Application of DNA-based diagnosis in detection of schistosomal DNA in early infection and after drug treatment. Parasites & Vectors. 2011, 4 : 164. www.parasitesandvectors.com/content/4/1/164-173. doi: 10.1186/1756-3305-4-164. diakses pada: 20 Maret 2015.

Kumagai T, Shimogawara R.F, Ohmae H, Wang T.P, et al. Detection of early and sigle infection of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and Loop-mediated isothermal amplification (LAMP) Assay. Am.J.Trop.Med.Hyg. 2010. 83(3): 542-548. doi: 10.4269/ajtmh.2010.10- 0016. diakses pada: 20 Maret 2015.

Sudjadi. Bioteknologi Kesehatan. Kanisius. Yogyakarta. 2008: 131-135.

Driscoll J, Kyle J.L, and Remais J. Development a novel assay capable of detecting a single Schistosoma japonicum cercaria recovered from Oncomelania hupensis. Parsitology. 2005.

: 497 - 500. d o i : 10.1017/S0031182005007961. diakses

pada: 19 Maret 2015.

Worrell C, Xiao N, Vidal J.E, Chen L, et al,. Field Detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR. Applied and Environmental Microbiology.2011. 11: 2192-2195. http: aem.asm.org. doi: 10.1128/AEM/01561-10. diakses pada: 19 Maret 2015.

Velasquez, L.E, R.L. Caldeira, V. Estrada and O.S Carvalho. Morphological and polymerase chain reaction-restriction fragment lenght

polymorphism characterization of Biomphalaria kuhniana and Biomphalaia

amazonia from Colombia. Mem. Inst. Oswaldo Cruz. 2002. 97: 997-1004. diakses pada: 19 Maret 2015.

Hamed, M.A. Strategic Control of Intermediate Host. Asian J. Epidemiol. 2010. 3 (3): 123-140. Diakses pada: 19 Maret 2015.

Janotti-Passos, L.K., K.G Magalhaes, O.S. Carvalho and T.H Vidigal. Multiplex PCR for both identification of Brazilian Biomphalaria

species (Gastropoda: Planorbidae) and diagnosis of infection by Schistosoma mansoni (Trematoda: Schistosomatidae). J. Parasitol. 2006. 85: 253-258. diakses pada: 19 Maret 2015.

Abdel-Hamid, Z.A., S.M. Rawi and A.F Arafa. Identification of genetic marker associated with the resistance to Schistosoma mansoni: Possible application in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection. Mem. Inst. Oswaldo Cruz. 2006. 101: 145-148. diakses pada: 19 Maret 2015.

Lockyer A.E., J. Sprinks, L.R. Noble, D. Rollinson and C.S. Jones. Identification of genes involved in interactions between Biomphalaria glabrata and Schistosoma mansoni by suppression subtractive hybridization. Mol. Biochem. Parasitol. 2007. 151: 18-27. diakses pada: 19 Maret 2015.

Hahn, U.K., R.C. Bender, J.K. Brooks and C.J. Bayne. Involvement of nitric oxide in killing of Schistosoma mansoni sporocysts by hemocytes from resistant Biomphalaria glabrata. J. Parasitol. 2001. 87: 778-785. diakses pada: 19 Maret 2015.

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