Aplikasi Teknik Diagnosis Schistosomiasis Berbasis Molekuler

Anis Nurwidayati

Abstract


Schistosomiasis ranks second only to malaria in a public health problem in the world.Schistosomiasis in Indonesia is endemic in Central Sulawesi, which is in Lindu, Napu andBada Highland. The disease is caused by trematodes worm, Schistosoma spp and requiresfreshwater snails as the intermediate host. The World Health Organization (WHO)recommends schistosomiasis research focus is the development and evaluation of newstrategies and tools for disease control. This paper aims to describe several moleculartechniques to detect schistosomiasis in human or intermediate snails. This paperarranged by compiled based search journals and scientific articles related moleculartechniques for detecting schistosomiasis using PCR and LAMP. Molecular basedtechniques for schistosomiasis diagnostic have been developed in several countries, suchas pcr and lamp. Several studies have compared the techniques of schistosomiasisdiagnosis in human by polymerase chain reaction (PCR) with the technique of loopmediatedisothermal amplification (LAMP). Several studies show the results of pcr andlamp applications to detect dna schistosoma on snails. Based on the results of variousstudies, it is known applications, advantages and disadvantages of each - each technique.

 

Schistosomiasis menempati urutan kedua setelah malaria dalam masalah kesehatanmasyarakat di dunia. Schistosomiasis di Indonesia ditemukan endemis di SulawesiTengah, yaitu di Dataran Tinggi Lindu, Napu dan Bada. Penyakit ini disebabkan olehcacing Trematoda, Schistosoma spp dan membutuhkan keong air tawar sebagai hospesperantaranya. Badan Kesehatan Dunia (WHO) merekomendasikan fokus penelitianschistosomiasis adalah pengembangan dan evaluasi strategi dan alat baru untukpengendalian penyakit. Tulisan ini bertujuan untuk mendeskripsikan beberapa teknikmolekuler untuk mendeteksi schistosomiasis baik pada manusia maupun keongperantaranya. Tulisan disusun berdasarkan penelusuran jurnal dan artikel ilmiahterkait teknik molekuler PCR dan LAMP untuk mendeteksi schistosomiasis. Teknikdiagnosis schistosomiasis berdasarkan molekuler telah banyak dikembangkan dibeberapa negara, diantaranya adalah PCR dan LAMP. Beberapa penelitianmembandingkan teknik diagnosis schistosomiasis dengan Polymerase Chain Reaction(PCR) dengan teknik Loop-Mediated Isothermal Amplification (LAMP). Beberapa studimenunjukkan hasil dari aplikasi teknik PCR dan LAMP untuk mendeteksi DNASchistosoma pada keong. Berdasarkan hasil dari berbagai penelitian tersebut, dapatdiketahui aplikasi, kelebihan dan kekurangan dari masing – masing teknik.


Keywords


Schistosomiasis, DNA, PCR, LAMP, Snails

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