ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF BANGLE (Zingiber cassumunar Roxb.) RHIZOME Aktivitas Antioksidan dan Kandungan Fenol Total Rimpang Bangle (Zingiber cassumunar Roxb.)

Lia Marliani, Winasih Rahmawati, Anju Sinurat

ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF BANGLE (Zingiber cassumunar Roxb.) RHIZOME Aktivitas Antioksidan dan Kandungan Fenol Total Rimpang Bangle (Zingiber cassumunar Roxb.)

Abstract


ABSTRACT Bangle (Zingiber cassumunar Roxb.) is one of aromatic plants that has long been used as traditional medicine. Cassumunar rhizome has many active compound which are prospective to be one of traditional complementary and alternative medicine. Those active compounds such as essential oils, terpenoids, curcuminoids, and phenolic compounds that has antioxidants, anti-inflammatory and anti-bacterial activity. The aims of this study was to determine antioxidant activity and total phenolic content of Z. cassumunar Roxb. rhizome. Determination of antioxidant activity was done by DPPH free radical scavenging method. Total phenolic content was determined using Spectrophotometry UV-Vis used the Folin Cio-calteu reagent. The results showed that Z. cassumunar Roxb. rhizome has moderate antioxidant activity (IC50 142 μg/ml). Total phenolic content of Z. cassumunar Roxb. rhizome was 2.71 g GAE/100 g extract or 0.30 g GAE/ 100 g crude drugs.Keywords: Zingiber cassumunar Roxb., antioxidant, DPPH, total phenolic contentVolume

 

ABSTRAK Bangle (Zingiber cassumunar Roxb.) merupakan salah satu tanaman aromatik yang telah lama digunakan sebagai obat tradisional. Rimpang bengle memiliki banyak senyawa aktif yang prospektif menjadi salah satu pengobatan komplementer dan alternatif tradisional, antara lain minyak esensial, terpenoid, kurkuminoid, dan senyawa fenolik yang memiliki antioksidan, aktivitas anti-inflamasi dan anti-bakteri. Tujuan dari penelitian ini adalah untuk menentukan aktivitas antioksidan dan kandungan total fenolik dari rimpang bengle. Penentuan aktivitas antioksidan dilakukan dengan metode radikal bebas DPPH. Total kandungan fenolik ditentukan dengan menggunakan Spektrofotometri UV-Vis menggunakan Folin Cio-calteu reagen. Hasil penelitian menunjukkan bahwa rimpang bangle memiliki aktivitas antioksidan sedang (IC50 142 mg / ml). Total kandungan fenolik dari Bangle Roxb. rimpang adalah 2.71 g GAE / 100 g ekstrak atau 0,30 g GAE / 100 g simplisia.


Keywords


Zingiber cassumunar, antioksidan, DPPH, kandungan fenol total.

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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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