SCREENING ACTIVE FRACTION OF ETHANOLIC EXTRACT OF PASAK BUMI (Eurycoma longifolia Jack) ROOT AS ANTIOXIDANT Skrining Fraksi Aktif Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia Jack) Sebagai Antioksidan

Vera Nurviana, Nurkhasanah Nurkhasanah, Laela Hayu Nurani

SCREENING ACTIVE FRACTION OF ETHANOLIC EXTRACT OF PASAK BUMI (Eurycoma longifolia Jack) ROOT AS ANTIOXIDANT Skrining Fraksi Aktif Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia Jack) Sebagai Antioksidan

Abstract


ABSTRACT Eurycoma longifolia Jack has been used as traditional medicines to cure many kinds of diseases. Previous researches reported to have antioxidant, antimalaria, anticancer, stamina enhancer, and antiangiogenesis activities. The objective of this research was screen active fraction as antioxidant from ethanolic extract of Eurycoma longifolia Jack. The ethanolic ectract was fractionated using n-hexan, ethyl acetate, and methanol. Antioxidant cctivity were observed using DPPH method. The results showed that all the treatments had the activity as scavenging the free radical, with the value ES50 ethanolic extract was (60,796 ± 0,051 μg/ml) fraction of n-hexan (237,621 ± 1,662 μg/ml) ethyl acetate fraction was (53,597±0,028 μg/ml), and methanolic fraction was (109,760±0,34 μg/ml). The best activity radical scavenging using DPPH method is ethyl acetate fraction.Key words: Eurycoma longifolia Jack., antioxidant, free radical, DPPH.

 

ABSTRAK Eurycoma longifolia Jack telah digunakan sebagai obat tradisional untuk menyembuhkan berbagai jenis penyakit. Tumbuhan tersebut dilaporkan mempunyai aktivitas sebagai antioksidan, antimalaria, antikanker, penambah stamina, dan antiangiogenesis pada beberapa penelitian. Tujuan dari penelitian ini adalah skrining fraksi aktif sebagai antioksidan dari ekstrak etanol Eurycoma longifolia Jack. Ekstrak etanol masing-masing difraksinasi menggunakan n-heksan, etil asetat, dan metanol. Aktivitas antioksidan yang diamati menggunakan metode DPPH. Hasil penelitian menunjukkan bahwa semua perlakuan memiliki aktivitas sebagai penangkap radikal bebas, adalah ekstrak etanol dengan nilai ES50 (60.796 ± 0.051 μg/ml) fraksi n-heksana (237.621 ± 1,662 μg/ml) fraksi etil asetat (53.597 ± 0.028 μg/ml), dan fraksi metanol (109760 ± 0,34 μg/ml). Aktivitas antioksidan tertinggi E. longifolia menggunakan metode DPPH adalah fraksi etil asetat.


Keywords


Eurycoma longifolia Jack., antioksidan, radikal bebas, DPPH.

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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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