CYTOTOXICITY EFFECT OF CINNAMON OIL ON WiDr & Vero CULTURE CELL AND SUB CRONIC TOXICITY EFFECT OF CINNAMON OIL ON ALT/AST LEVELS MALE RAT Efek Sitotoksisitas Minyak Kayu Manis Terhadap Kultur Sel WiDr & Vero Serta Efek Toksisitas Subkronis Minyak Kayu Man

W Herdwiani, F Leviana, Z Imama, Sari Sari, AA Soemardji, Elfahmi Elfahmi, MI Tan

CYTOTOXICITY EFFECT OF CINNAMON OIL ON WiDr & Vero CULTURE CELL AND SUB CRONIC TOXICITY EFFECT OF CINNAMON OIL ON ALT/AST LEVELS MALE RAT Efek Sitotoksisitas Minyak Kayu Manis Terhadap Kultur Sel WiDr & Vero Serta Efek Toksisitas Subkronis Minyak Kayu Man

Abstract


ABSTRACTMore than 1 million cancer cases occur each year and half are in developing countries. Attention towards new alternative was invited by the failure of conventional chemotheraphy. The present work has addressed the antineoplastic potential of the Cinnamon Oil in cancer. This research aims to investigate cytotoxity effect of Cinnamon Oil On colorectal cancer culture cells (WiDr) and normal Culture Cell (Vero) and subchronic toxicity effect of cinnamon oil On Male Rat. Sitotoxic effect of WiDr culture cell and Vero culture cell were done by MTT assay, to get an IC50 number and subchronic toxicity testing using 20 male rat and were divided into 4 groups. Each group was given treatment of cinnamon oil in difference doses, first group 50 mg/kg BW, second group 100 mg/kg BW, third group 200 mg/kg BW and the last group negative control. The data were obtained from the examination SGPT/ALT nad SGOT/AST before and after treatment on 1st, 2nd, 3rd and 4th week and histopathological figures of the hepar at the end of 4th week. The data results were analyzed using SPPS. The results showed that cinnamon oil had an IC50 of WiDr and Vero culture cell 38,02 μg/ml and 27,35 μg/ml respectively. Cinnamon oil had no subchronic toxicity effect on the hepar of white male rat.Keywords : cinnamon, citotoxicity, WiDr, Vero, toxicityVolume

 

ABSTRAKLebih dari 1 juta kasus kanker terjadi setiap tahun dan separuhnya berada di negara-negara berkembang. Pengembangan pengobatan alternatif pada kanker saat ini menjadi perhatian penting dikarenakan kegagalan kemoterapi konvensional. Karya ini telah membahas potensi antineoplastik Minyak Cinnamon kanker. Penelitian ini bertujuan untuk mengetahui efek cytotoxity dari Cinnamon Oil On sel kolorektal budaya kanker (WiDr) dan normal Kultur Sel (Vero) dan efek toksisitas subkronis minyak kayu manis Pada Pria Rat. Efek Sitotoxic dari WiDr sel kultur dan budaya Vero sel dilakukan oleh MTT assay, untuk mendapatkan nomor IC50 dan pengujian toksisitas subkronis menggunakan 20 tikus jantan dan dibagi menjadi 4 kelompok. Setiap kelompok diberi perlakuan minyak kayu manis dalam dosis perbedaan, pertama kelompok 50 mg / kg BB, kelompok kedua 100 mg / kg BB, kelompok ketiga 200 mg / kg BB dan kelompok terakhir kontrol negatif. Data diperoleh dari pemeriksaan SGPT / ALT nad SGOT / AST sebelum dan sesudah perlakuan pada tanggal 1, 2, minggu ke-3 dan ke-4 dan tokoh histopatologi hepar pada akhir minggu ke-4. Hasil Data dianalisis dengan menggunakan SPPS. Hasil penelitian menunjukkan bahwa minyak kayu manis memiliki IC50 WiDr dan Vero sel kultur 38,02 mg / ml dan 27,35 mg / ml masing-masing. Minyak Cinnamon tidak memiliki efek toksisitas subkronis pada hepar tikus putih jantan.


Keywords


kayu manis, sitotoksisitas, WiDr, toksisitas

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CYTOTOXICITY EFFECT OF CINNAMON OIL ON WiDr & Vero CULTURE CELL AND SUB CRONIC

TOXICITY EFFECT OF CINNAMON OIL ON ALT/AST LEVELS MALE RAT

Efek Sitotoksisitas Minyak Kayu Manis Terhadap Kultur Sel WiDr & Vero

Serta Efek Toksisitas Subkronis Minyak Kayu Manis Pada Kadar ALT/ASL Tikus Putih Jantan


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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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