UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAN FRAKSI DAUN KEMBANG DAYANG (Cestrum nocturnum L.) Antioxydant activity of extract and fraction of kembang dayang (Cestrum nocturnum L.)

Soraya Riyanti, Julia Ratnawati, Resti Fauziah

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAN FRAKSI DAUN KEMBANG DAYANG (Cestrum nocturnum L.) Antioxydant activity of extract and fraction of kembang dayang (Cestrum nocturnum L.)

Abstract


ABSTRACTAntioxidants are compounds that can scavenge free radicals and highly reactive molecules, which can inhibit celldamage. Kembang dayang leaves contains phenolic compounds and flavonoids that thought to be able to act asfree radical semuevenger. This study aims to determine the antioxidant activity of the ethanolic extract, fractionn-hexanic, ethyl acetate fraction and water fraction of kembang dayang leaves. Dried leaves were extractedby maceration using ethanol solvent followed by fractionation with liquid-liquid extraction using n-hexane,ethyl acetate and water. Antioxidant activ2-picrylhydrazyl) using UV-Visible spectrophotometry and quercetin as standard. The result of phytochemicalscreening showed kembang dayang leaves (Cestrum nocturnum L.) contains saponins, tannins, polyphenols,flavonoids, monoterpenoid/sesquiterpenoid, steroids/triterpenoids. The results of the antioxidant activityexamination of ethanolic extract, fraction n-hexanoid, ethyl acetate fraction and water fraction of kembangdayang leaves showed the EC50 of 53.17 mg/ml; 65.56 mg/ml; 23.74 mg/mL and 98.25 ug/ml respectively,whereas quercetin showed the EC50 value 4.18 mg/ml.

 

ABSTRAKAntioksidan merupakan senyawa yang dapat mengikat radikal bebas dan molekul yang sangat reaktif,sehingga dapat menghambat terjadinya kerusakan sel. Daun kembang dayang mengandung senyawa fenoldan flavonoid yang diduga dapat berperan sebagai senyawa penangkap radikal bebas. Penelitian ini bertujuanuntuk mengetahui aktivitas antioksidan ekstrak etanol, fraksi n-heksana, fraksi etil asetat dan fraksi air daunkembang dayang. Simplisia daun kembang dayang diekstraksi dengan cara maserasi menggunakan pelarutetanol dilanjutkan dengan fraksinasi dengan cara ekstraksi cair-cair menggunakan pelarut n-heksana, etilasetat dan air. Pengujian aktivitas antioksidan ditentukan dengan menggunakan pereaksi DPPH (1,1-difenil-2-pikrilhidrazil) menggunakan spektrofotometri UV-Visible dan kuersetin sebagai pembanding.Hasil penapisanfitokimia menunjukkan daun kembang dayang (Cestrum nocturnum L.) mengandung saponin, tanin, polifenol,flavonoid, monoterpen/sesquiterpeniod, steroid/triterpenoid. Hasil pengujian aktivitas antioksidan ekstraketanol, fraksi n-heksana, fraksi etil asetat dan fraksi air daun kembang dayang dengan EC50 berturut-turut53,17 μg/ml; 65,56 μg/ml; 23,74 μg/ml dan 98,25 μg/ml, sedangkan nilai EC50 isolat kuersetin adalah 4,18 μg/ml.Kata kunci: daun kembang dayang (Cestrum nocturnum L.), kuersetin, fraksi, DPPH.


Keywords


Keywords: kembang dayang leaves (Cestrum nocturnum L.), quersetin, fractions, DPPH.ity examination was determined using reagent DPPH (1,1-dipheny

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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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