Correlation between Cytotoxic Activity on MCF-7 Cell and Total Steroid Level on Trigonella foenum-graecum L.

Kurnia Agustini, Wahono Sumaryono, Azri Fitria

Correlation between Cytotoxic Activity on MCF-7 Cell and Total Steroid Level on Trigonella foenum-graecum L.

Abstract


ABSTRACT

Trigonella foenum-graecum (TFG) is one of medicinal plants containing some steroidal sapogenins such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also alkaloid trigonellin, which has many activity as antidiabetic, estrogenic and also as anti cancer. The aim of this research is to investigate the correlation of its cytotoxic activity on breast cancer cell line, MCF-7, with its total steroid level. Samples was prepared by fractioned the methanolic TFG with ethylacetate, n-hexane and n-buthanol. Each fraction then was separated by chromatographic coloumn. Cytotoxic activity was investigated using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium) assay on MCF-7 cell, while total steroid level was done using spectrophotometric methods. Results showed that there was no correlation between cytotoxic activity and total steroid level. Thus we can conclude that the cytotoxic effect from TFG is not caused by its steroid compounds.

ABSTRAK

Trigonella foenum-graecum L. (TFG) adalah salah satu  tumbuhan obat yang mengandung beberapa steroid sapogenin seperti diosgenin, yamogenin, gitogenin, tigogenin, dan trigoneosid, serta alkaloid trigonellin. TFG memiliki aktivitas antidiabetik, estrogenik, dan antikanker. Penelitian ini bertujuan untuk menguji korelasi ak-tivitas sitotoksik TGF pada sel kanker payudara MCF-7 serta kadar steroid total. Ekstrak metanol TGF difraksi-nasi dengan etil asetat, n-heksan, dan n-butanol. Tiap fraksi dipisahkan dengan kromatografi kolom. Aktivitas sitotoksik pada sel MCF-7 diuji dengan uji MTT [3-(4,5-dimetiltiazol-2-il)2,5-difeniltetrazolium]. Kadar ste-roid total ditetapkan dengan metode spektrofotometri. Hasil penelitian menunjukkan tidak terdapat korelasi antara aktivitas sitotoksik dengan kadar steroid total. Sehingga dapat disimpulkan bahwa efek sitotoksik TFG bukan merupakan kontribusi dari komponen steroid.


Keywords


Key words: Trigonella foenum graecum L., MTT, MCF-7, total steroid level.

References


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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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