AKTIVITAS INFUSA DAUN Piper betle Linn DAN Piper crocatum Ruiz & Pav TERHADAP VIABILITAS SEL HeLa Activity of the infusion of Piper betle L. and Piper crocatum Ruiz & Pav on HeLa cell line viability

Sari Haryanti, Yuli Widiyastuti, Nita Etikawati

AKTIVITAS INFUSA DAUN Piper betle Linn DAN Piper crocatum Ruiz & Pav TERHADAP VIABILITAS SEL HeLa Activity of the infusion of Piper betle L. and Piper crocatum Ruiz & Pav on HeLa cell line viability


ABSTRACTPiper betle L. (betel leaves) and red betel (Piper crocatum Ruiz & Pav) are medicinal plant that are often usedby the Indonesian people to treat various diseases, including cancer. Scientific evidence to strengthen then useas anticancer is still necessary. This study was conducted to determine the effect of green and red betel leafinfusion against HeLa cervical cancer cell viability, as well as its potential to inhibit the expression of Bcl2 protein qualitatively. Infusionof betel leaves were dried over a water bath to obtain a dry extract. Effect of extracts on theviability of HeLa cells determined by the cytotoxic test with MTT assay method to obtain IC50 values . Effect theactivity against Bcl2 protein was tested byimmunocyitochemical test. Cytotoxic test infution of green betel leavesgave IC50 value of 49.14 ug/ml, whereas the red betel showed no cytotoxic effects. The immunocytochemical testshowed a reduction in Bcl-2 protein expression.The results of this study showed that green betel leaves affect HeLacell viability by inducing cell death through inhibition of anti-apoptotic Bcl2 protein expression. Thus green betelleaves are potential to be developed as a chemopreventive agent.Keywords: Piper betle, Piper crocatum, cytotoxic, HeLa cells


ABSTRAKSirih hijau(Piper betle L.) dan sirih merah (Piper crocatum Ruiz & Pav) merupakan tanaman yang seringdimanfaatkan oleh masyarakat Indonesia untuk mengobati berbagai macam penyakit, termasuk kanker. Buktiilmiah untuk memperkuat dasar penggunaannya sebagai antikanker masih sangat diperlukan. Penelitian inidilakukan untuk mengetahui efek infusa daun sirih hijau dan sirih merah terhadap viabilitas sel kanker serviksHeLa, serta potensinya dalam menghambat ekspresi protein antiapoptosis Bcl2 secara immunositokimia.Infusa sirih dikeringkan di atas penangas air hingga diperoleh ekstrak kering. Pengaruh ekstrak terhadapviabilitas sel HeLa ditetapkan melaluiuji sitotoksik dengan metode MTT assay hingga diperoleh nilai IC50.Pengaruh ekstrak aktif terhadap protein Bcl2 dilakukan dengan pengecatan immunositokimia. Uji sitotoksikinfusa sirih hijau memberikan nilai IC50 49.14 μg/ml, sedangkan sirih merah tidak menunjukkan efek sitotoksik.Hasil pengecatan immunositokima secara kualitatif memperlihatkan pengurangan ekspresi protein Bcl2.Hasil penelitian ini menunjukkan bahwa sirih hijau mempengaruhi viabilitas sel HeLa dengan menginduksikematian sel melalui penghambatan ekspresi protein antiapoptosis Bcl2. Dengan demikian sirih hijau cukuppotensial dikembangkan sebagai agen kemopreventif untuk kanker serviks.



Abrahim NN., Kanthimathi MS. dan Abdul-Aziz

A. 2012. Piper betle shows antioxidant

activities, inhibits MCF-7 cell proliferation

and increases activities of catalase and

superoxide dismutase. BMC Complementary

and Alternative Medicine, 12:220

Alfarabi M., Bintang M., Suryani, dan Safithri

M. 2010. The Comparative Ability of

Antioxidant Activity of Piper crocatum in

Inhibiting Fatty Acid Oxidation and Free

Radical Scavenging. Hayati J Biosci., 17(4):


Bachrah ZY., 2012. Contribution of Selected

Medicinal Plants for Cancer Prevention and

Therapy. Scientific Journal of the Faculty of

Medicine in Niš., 29(3): 117-123

Batubara I., Rahminiwati M., Darusman LK. dan

Mitsunaga T. 2011. Tyrosinase activity of

Piper betle and Piper crocatum Essential Oil.

Proceedings of The International Conference

on Basic Science. Faculty of Science.

University of Brawijaya. Malang Indonesia.

Datta A., Ghoshdastidar S. dan Singh M. 2011.

Antimicrobial Property of Piper betel

leaf against Clinical Isolates of Bacteria.

International Journal of Pharma Sciences

and Research (IJPSR), 2(3): 104-109

Hajare R., Darvhekar VM., Shewale A. dan Patil V.

Evaluation of antihistaminic activity

of Piper betel leaf in guinea pig. Afr. J. Pharm.

Pharmacol., 5(2): 113-117,

Hanahan D. and Weinberg RA. 2011. Hallmarks

of Cancer: The Next Generation. Cell,144:


Hetz CA., Torres V. and Quest AVG. 2005. Beyond

Apoptosis: Nonapoptotic Cell Death in

Physiology and Disease. Review. Biochem.

Cell Biol., 83(5): 579–588.

Nazari Z., Behtash N., Gilan MM., Ganjoei TA. 2007.

Cervical carcinoma simulating advanced

ovarian cancer. Eur. J. Surg. Oncol., 33(1):


Patel G., Patil UK., and Devi PU. 2012. Preliminary

Study on the Effect of Piper betle on the

Growth of Transplanted B16F10 Melanoma

in Mice. Int J Recent Adv Pharm Res., 2(1):67-

Pin KY., Chuah TG., Abdull Rashih A., Rasadah

MA., Law CL., and Choong TSY.. 2006.

Aqueous Extraction of Hydroxychavicol

from Piper Betle L. Leaves. Proceedings of

the 1st International Conference on Natural

Resources Engineering & Technology.

Putrajaya, Malaysia, 146-152

Safithri M. and Fahma F. 2008. Potency of Piper

crocatum Decoction as an Antihiperglycemia

in Rat Strain Sprague dawley. Hayati J

Biosci., 15(1): 45-48

Singh GK., Azuine RE., and Siahpush M. 2012.

Global Inequalities in Cervical Cancer

Incidence and Mortality are Linked to

Deprivation, Low Socioeconomic Status,

and Human Development. International

Journal of MCH and AIDS, I(1): 13-26

Tsao AS., Kim ES., Hong WK., 2004.

Chemoprevention of Cancer. Cancer J Clin.,


Tzifi F., Economopoulus C., Gourgiotis D.,

Ardavanis A., Papageorgiou S., and Scorilas

A. 2012. The Role of BCL2 Family of

Apoptosis Regulator Proteins in Acute and

Chronic Leukemias. Advances in Hematology

: 524308

Wicaksono BD., Handoko YA., Arung ET, Kusuma

IW, Yulia D., Pancaputra AN. and Sandra

F. 2009. Antiproliferative Effect of the

Methanol Extract of Piper crocatum Ruiz &

Pav Leaves on Human Breast (T47D) Cells

In-vitro. Trop J Pharm Res., 8(4): 345-352

Full Text: PDF


  • There are currently no refbacks.

Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

Indexed by:

Lisensi Creative Commons
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Visitor number: View My Stats