Kekerabatan Filogenetik Buah Makassar (Brucea javanica) Berdasar - kan Gen Ribulosa -1,5-bifosfa T Karboksilase /Oksigenase Phylogenetic relationship of Brucea javanica based on molecular marker ribulose- 1,5-biphosphate carboxylase/oxyigenase gene

Tri Widayat, Dyah Subositi

Kekerabatan Filogenetik Buah Makassar (Brucea javanica) Berdasar - kan Gen Ribulosa -1,5-bifosfa T Karboksilase /Oksigenase Phylogenetic relationship of Brucea javanica based on molecular marker ribulose- 1,5-biphosphate carboxylase/oxyigenase gene

Abstract


ABSTRACTBuah makassar (Brucea javanica) is one of medicinal plant collections in Medicinal plant and Traditional MedicineResearch Center (B2P2TO-OT) Tawangmangu. Phyllogenetic relationship data of B. javanica is important informationas a database for further research. The objective of this study was to provide phylogenetic relationshipof B. javanica based on molecular marker ribulose-1,5-biphosphate carboxylase/oxyigenase gene(rbcL). The rbcLsequence in this research was taken from National Center for Biotechnology Information (NCBI) and the sequencewas aligned by using ClustalW program to construct phylogenetic tree. The result showed that B. javanica has aclosed relationship with some species of Anacardiaceae.Key words: Brucea javanica, phylogenetic relationship, rbcL

 

ABSTRAKBuah makassar (Brucea javanica) adalah salah satu tanaman obat yang dikoleksi Balai Besar Penelitian danPengembangan Tanaman Obat dan Obat Tradisional (B2P2TO-OT), Tawangmangu. Salah satu aspek daritanaman obat tersebut yang datanya belum terkoleksi di B2P2TO-OT adalah kekerabatan. Oleh karena itu,tujuan dari studi kekerabatan B. javanica adalah untuk mengetahui hubungan kekerabatan buah B.javanicaberdasarkan penanda molekuler gen ribulosa-1,5-bifosfat karboksilase/oksigenase (rbcL). Hubungan kekerabatantersebut dapat dilihat dari pohon filogeni yang dkonstruksi berdasarkan sequence nukleotida pada rbcL.Sequence rbcL diperoleh dari National Center for Biotechnology Information (NCBI) dan aligment sequence untukkonstruksi pohon filogenetik menggunakan program ClustalW yang diakses dari DNA Data Bank of Japan(DDBJ) secara online. Pohon filogenetik menunjukkan bahwa B. javanica berkerabat dengan beberapa spesiestanaman anggota familia Anacardiaceae.


Keywords


Brucea javanica, filogenetik, rbcL

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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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