Cytotoxicity Effect of Zingiber officinale, Curcuma aeruginosa and Curcuma xanthorhiza Extracts on Adipose-Derived Stem Cells (ADSCs)

Rilianawati Rilianawati, Mutia Hardhiyuna, Angelia Yulita, Ago Halim


In the past few decades, attention and research in the field of stem cell are progressing very rapidly. Hospitals in Indonesia have been using stem cells as an alternative to cure some illnesses like diabetes, heart disease, fractures and joints, dental implants, and asthma. The purpose of this research was to characterized and to produced stem cells from human subcutaneous adipose tissue (ADSC), which can be used for assessing cytotoxicity effects potency of Zingiber officinale (red ginger), Curcuma aeruginosa (temu ireng) and Curcuma xanthorhiza (temulawak) on ADSC proliferation. The cytotoxic effect was done using MTT assay. The results showed Z. officinale var. and C. xanthorhiza Roxb. extracts have no cytotoxic effect to ADSC at 25 ppm, 50 ppm and 100 ppm concentrations, however the proliferation inhibition of Z. officinale extracts gradually rises to 0.05 % at 200 ppm and slightly fall at 600 ppm, after that it sharply raises unto 74.84% at 1000 ppm. The proliferation inhibition of C. xanthorhiza extracts at 200 ppm is about 28% and significantly increase to 90 % at 1000 ppm which is the highest percentage on the figure. The C. aeruginosa extracts has no inhibition effect to ADSC from 25 ppm until 800 ppm concentration of extract, however it modestly goes up at 1000 ppm reaching 9% of inhibition. Further studies are required to investigate the metabolites in these extracts that are highly correlated with its toxic effects.


adipose derived stem cells, adherent stem cells, cytotoxicity, plant extract



Fraser JK, Wulur I, Alfonso Z, Hedrick MH. 2006. Fat Tissue: An Underappreciated Source of Stem Cells for Biotechnology. Trends Biotechnol. 24: 150–154.

Kuhbier JW, Weyand B, Radtke C, and Vogt PM. 2010. Isolation, Characterization, Differentiation, and Application of Adipose-Derived Stem Cells. Adv Biochem Engin/Biotechnol. 123:55-105

Kirana, Chandra. 2003. Potential Anticancer Activity in Rhizomes of Ginger Species (Zingiberaceae family). Thesis. University of Adelaide, Dept of Medicine & Dept. of Horticulture, Viticulture and Oenology. Australia.

Kitagawa Y, Korobi M, Toriyama K, Kamei Y, Torii S. 2006. History of Discovery of Human Adipose-Deived Stem Cells and Their Clinical Applications. Jpn J Plast Reconstr Surg. 49:1097–1104.

PA Batugal, J Kanniah, Lee SY and JT Oliver, editors. 2004. Medicinal Plant Research in Asia, volume I: The Framework and Project Work. International Plant Genetic Resources Institute.

R. A. Musina, E. S. Bekchanova, A. V. Belyavskii*, and G. T.

Sukhikh. 2006. Differentiation Potential of Mesenchymal Stem Cells of Different Origin. Cell Technologies in Biology and Medicine, 2(1):147-151.

Safety Data Sheets (SDS), Certificates of Analysis. 2013. Life Technologies Corporation.

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Jurnal Tumbuhan Obat Indonesia (Journal of  Indonesian Medicinal Plant, p-ISSN : 1979-892X, e-ISSN : 2354-8797) is published by Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional (B2P2TOOT) Tawangmangu.

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