FRAKSI ETANOL 96% BIJI KORO BENGUK (Mucuna pruriens L.) SEBAGAI PENINGKAT KUALITAS SPERMATOZOA MENCIT (Mus musculus)

Sri Winarni, Rina Judiwati, Bambang Prajogo, Alfiah Hayati

FRAKSI ETANOL 96% BIJI KORO BENGUK (Mucuna pruriens L.) SEBAGAI PENINGKAT KUALITAS SPERMATOZOA MENCIT (Mus musculus)

Abstract


Background: The examination of sperm quality is the main priority for infertility diagnosis. Based on previous study with mice, active ingredient of Mucuna pruriens L. or koro benguk (Papilionaceae), the L-dopa, may affect the quality of spermatozoa.

Objective: Research was to study the effect of 96% ethanol fraction Mucuna pruriens seed on spermatozoaquality of mice exposed to 2-Methoxy ethanol. L-dopa in 96% ethanol fraction of M. pruriens seed was 14.7%.

Methode: This was an experimental study using complete randomized design. Subjects were BALB/C mice (Mus musculus). Five groups served as control, 3 groups received subcutaneos injection of 2-ME as muchas 100 mg/kg.bw/day for 12 days, followed with 96% ethanol fraction Mucuna pruriens seed starting from14 mg/kg.bw/day, 28 mg/kg.bw/day, and 56 mg/kg.bw/day for 51 days.

Result: The 96% ethanol fraction of Mucuna pruriens seeds are significant increase motility (p<0,01) andthe percentage of normal spermatozoa morphology (p= 0,042).

Conclusion: 96% ethanol fraction of Mucuna pruriens seeds are able to increase motility and the percentage of normal spermatozoa morphology in mice exposed to 2-ME.

 

Keywords: Mucuna pruriens L., L-dopa, mouse spermatozoa


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