PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

Dyah Widiastuti, Sitti Rahmah Umniyati, Nastiti Wijayanti

PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

Abstract


ABSTRACT
Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected
Aedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main target
of surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an early
warning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash using
immunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for dengue vector
surveillance. The study aimed to develope immunocytochemical SBPC assay to detect Dengue virus infection in head
squash of Ae.aegypti. The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3
were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. The
immunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash to
detect Dengue virus antigen. The results were analyzed by descriptive analysis. The immunocytochemical SBPC assay
can detect Dengue virus antigen in mosquito head squash at day 2 postinfection. There are some false positive results
found in immunocytochemical SBPC assay.
Key Word: Dengue, immunocytochemistry, DSSE10

Full Text: PDF

Refbacks

  • There are currently no refbacks.


BALABA was indexed by:

Visitor Number : View My Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License