Aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang

Dyah Widiastuti, Sunaryo Sunaryo, Nova Pramestuti, Martini Martini

Abstract


Abstract. Dengue Haemorrhagic Fever (DHF) is a major health problem in Tembalang sub district, Semarang City. Fogging with insecticide applications was done frequently as an effort to control Dengue vectors. The use of insecticides from the same class in a long time can lead to resistance in mosquitos’ population. The research aimed to observe the activity of monooxygenases in Aedes aegypti populations in Tembalang Subdistrict, Semarang. The study was conducted during February-November 2014 with a cross-sectional design in 10 villages in Tembalang Subdistirict, Semarang City. Field strains of Ae. aegypti eggs were collected using ovitraps. The collected eggs were grown under standard condition to adult mosquitoes. Mosquitos’ homogenate were stored at -85°C and used for biochemical assays. The results showed there was increased monooxygenases activity in Ae. aegypti populations. Resistance to synthetic pyrethroid insecticide in Ae. aegypti mosquitoes population in Tembalang Subdistrict might be caused by the mechanism of detoxification enzymes in particular monooxygenases

Keywords: monooxygenase, insecticide, Ae. aegypti, resistance

 

Abstrak. Demam Berdarah Dengue (DBD) masih menjadi masalah kesehatan utama di Kecamatan Tembalang, Kota Semarang. Tindakan fogging untuk pengendalian vektor DBD sering dilakukan. Penggunaan insektisida dari golongan yang sama dalam waktu cukup lama dapat memicu terjadinya resistensi. Tujuan penelitian untuk mengamati aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang. Penelitian dilaksanakan bulan Februari-November 2014 dengan desain potong lintang di 10 desa di Kecamatan Tembalang, Kota Semarang. Pada penelitian ini dilakukan pemasangan ovitrap untuk mendapatkan sampel telur yang dipelihara menjadi nyamuk dewasa. Sampel homogenate nyamuk disimpan pada suhu -85°C, selanjutnya dilakukan peng-ujian resistensi dengan uji biokimia untuk melihat aktivitas enzim monooksigenase. Hasil pengujian menunjukkan terdapat peningkatan aktivitas enzim monooksigenase. Resistensi terhadap insektisida sintetik piretroid pada populasi nyamuk Ae. aegypti di Kecamatan Tembalang disebabkan oleh mekani-sme peningkatan enzim detoksifikasi khususnya monooksigenase.

Kata Kunci: monooksigenase, insektisida, Ae. aegypti, resistensi

Keywords


monooxygenase; insecticide; Ae. aegypti; resistance

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Jurnal Aspirator (Journal of Vector-borne Disease Studies, p-ISSN: 2085-4102 e-ISSN: 2338-7343) is published by Badan Penelitian dan Pengembangan Kesehatan, Ministry of Health of Republic of Indonesia.

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