Efektivitas Pentagamavunon-0 (PGV-0) pada fase awal infeksi virus Dengue-2

Dewi Marbawati, Sardjiman Sardjiman, Risfah Yulianti

Efektivitas Pentagamavunon-0 (PGV-0) pada fase awal infeksi virus Dengue-2

Abstract


Abstract. Dengue virus infects 50 to 100 million people every year, however, specific treatment or effective antiviral drugs to treat viral infections has not been found yet. Curcumin known has  perform the inhibition of ubiquitin-proteasome system that causes a decrease of Japanese encephalitis, one kind of flavivirus. Structural modifications was known to increase the biological activity of curcumin. Pentagamavunon-0 (PGV-0) is known have activity similar to or even better than curcumin. This study aims to determine the effect of PGV-0 in the early phase of infection of dengue- virus 2 (one day of infection). This study includes quasi-experimental study. The method used for the detection of Dengue-2 viruswas immunocytochemistry, whichpreviously tested by PGV-0 cytotoxic test against vero cells. Cytotoxic test results indicate safe concentrations (no toxic effects) of PGV-0 against vero cells is 4.44 µM. Calculation of positive rate compared with the positive control(14.55 ± 7.25) showed that the value of positive rate due to one-day Dengue virus-2 infection with PGV-0 treatment was smaller(3.8 ± 3.89). It was concluded that the PGV-0 is able to decrease the positive rate due to Den-2 infection in the initial period of infection.

Keywords: dengue, Pentagamavunon-0 (PGV-0),immunocytochemistry, vero cells

Abstrak.Virus Dengue menginfeksi 50 sampai 100 juta orangper tahun, namun terapi yang spesifik atau obat antivirus yang efektif belum ditemukan. Kurkumin diketahui mampu melakukan penghambatan system ubiquitin-proteasome yang menyebabkan penurunan produksi salah satu jenis Flavivirus yaitu Japanese encephaitis. Modifikasi struktur kurkumin terbukti meningkatkan aktivitas biologisnya.  Pentagamavunon-0 (PGV-0) diketahui memiliki aktifitas mirip atau bahkan  lebih baik dari kurkumin. Tujuan dari penelitian ini adalah mengetahui pengaruh pemberian PGV-0 pada fase awal infeksi virus Dengue-2 (satu hari infeksi). Penelitian ini termasuk penelitian kuasi-eksperimental. Metode yang digunakan untuk deteksi virus Dengue-2 dalam penelitian ini adalah imunositokimia, setelah sebelumnya dilakukan uji sitotoksik PGV-0 terhadap sel vero. Hasil uji sitotoksik menunjukkan konsentrasi yang aman (tidak memberikan efek toksik) dari PGV-0 terhadap sel Vero adalah 4,44 µM. Hasil uji imunositokimia menunjukkan nilai rata-rata sel positif akibat infeksi Dengue-2 satu hari dengan perlakuan PGV-0 jauh lebih kecil (3.8 ± 3.89) dibandingkan kontrol positifnya (14.55 ± 7.25). Disimpulkan bahwa PGV-0 mampu menurunkan positive rate akibat infeksi Den-2 pada periode awal infeksi.

Kata Kunci: dengue, Pentagamavunon-0 (PGV-0), imunositokimia, sel vero


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Jurnal Aspirator (Journal of Vector-borne Disease Studies, p-ISSN: 2085-4102 e-ISSN: 2338-7343) is published by Badan Penelitian dan Pengembangan Kesehatan, Ministry of Health of Republic of Indonesia.

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