IDENTIFIKASI TIPE HLA KELAS II DENGAN TEKNIK PCR

Ervi Salwati

IDENTIFIKASI TIPE HLA KELAS II DENGAN TEKNIK PCR

Abstract


HLA (Human Leukocyte Antigen) contains a set of genes located together on the short arm of chromosome 6. These genes control immune responses, graft acceptance or rejection and tumor surveillance. These abilities have close relationship with genetic variation (occur in "many forms" or alleles) that bind and present antigens to T lymphocytes.

Using advanced technology and molecular biology approaches (PCR technique) detection of genetic variation in the HLA region (or HLA typing) has been performed based on DNA.. PCR is an in vitro technique to amplify the DNA sequence enzymatically. "Sequence Specific Primers" (SSP) are designed for this PCR to obtain amplification of specific alleles or groups of alleles. The PCR products are visualized through agarose gel electrophoresis stained with ethidium bromide.

The PCR technique requires small amount of whole blood (0.5 - 1 ml), gives rapid, accurate and complete result. This paper discuss identification of HLA class II typing using PCR-SSP technique and show the examples of the results.

 

Key words: HLA (Human Leukocyte Antigen) class II, PCR (Polymerase Chain Reaction)


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Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development)Indexed on:

p-ISSN: 0853-9987
e-ISSN: 2338-3445
Published by: Badan Penelitian dan Pengembangan Kesehatan
Jalan Percetakan Negara No.29
Jakarta Pusat 10560
Indonesia



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