Diagnosis Schistosomiasis dengan Metode Dot Blot

Samarang Samarang, Made Agus Nurjana, Malonda Maksud, Phetisya Pamela Frederika Sumolang, Gunawan Gunawan

Abstract


The diagnosis of schistosomiasis to detect secretory excretory antigen (AgES) Schistosoma japonicum by ELISA method, has been developed since 2012 through laboratory tests with good result, so it is necessary to do field test. Based on this the researchers conducted field tests, of schistosomiasis with dot blot in Napu, Poso district, Central Sulawesi Province. This study was conducted for nine months (March-November 2014) with cross sectional design. This study aims to determine the sensitivity and specificity of ELISA developed (dot blot). Fecal and blood surveys were conducted in communities in selected endemic villages, using simple random sampling method, with a total sample of 325 people. Furthermore, the blood serum tested using ELISA with dot blot technique. The sensitivity and specificity of the blot technique was calculated againts the results of microscopic examination (gold standard). Field test result showed the sensitivity and specificity of dot blot was 74% and 78%. Conclusion dot blot technique can detect Schistosomiasis on a laboratory scale or on a field scale.

Abstrak

Diagnosis schistosomiasis untuk mendeteksi antigen ekskretori sekretori (AgES) Schistosoma japonicum (S. japonicum) dengan metode ELISA, telah dikembangkan sejak tahun 2012 melalui uji laboratorium dengan hasil cukup baik, sehingga perlu dilakukan uji lapangan. Berdasarkan hal tersebut peneliti melakukan uji lapangan diagnosis schistosomiasis dengan dot blot di Napu, Kabupaten Poso, Provinsi Sulawesi Tengah. Penelitian dilaksanakan selama sembilan bulan (Maret - November 2014) dengan desain cross sectional. Penelitian ini bertujuan untuk menentukan sensitivitas dan spesifisitas dot blot yang dikembangkan. Survei tinja dan darah dilakukan pada masyarakat di desa endemis terpilih dengan metode simple random sampling, dengan jumlah sampel sebanyak 325 orang. Selanjutnya serumnya diuji ELISA dengan teknik dot blot. Sensitivitas dan spesifisitas teknik dot blot dihitung terhadap hasil pemeriksaan mikroskopis (gold standard). Hasil uji lapangan menunjukkan nilai sensitivitas dan spesifisitas dari teknik dot blot adalah sebesar 74% dan 78%. Kesimpulan teknik dot blot dapat mendeteksi schistosomiasis pada skala laboratorium maupun pada skala lapangan.


Keywords


Schistosomiasis; Dot blot; sensitivitas; spesifisitas.

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Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development)Indexed on:

p-ISSN: 0853-9987
e-ISSN: 2338-3445
Published by: Badan Penelitian dan Pengembangan Kesehatan
Jalan Percetakan Negara No.29
Jakarta Pusat 10560
Indonesia



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