Aktivitas Sitotoksik pada Sel MCF-7 dari Tumbuhan Indonesia untuk Pengobatan Tradisional Kanker Payudara

Sari Haryanti, Yuli Widiyastuti

Abstract


Indonesia is rich in a variety of potential plants as research materials based on local wisdom in the discovery of new medicinal compound. The research results have been documented in the research office of plant and herbal medicine (Ristoja) 2012. This research is a further assessment of Ristoja 2012, aims to test the cytotoxic activity of 20 parts of plants that are used for traditional treatment of cancer in Indonesia. The extraction was carried out by the infuse method using water solvent then dried in 40o C oven to obtain a dry extracts. The cytotoxicity activity test was performed on MCF-7 breast cancer cell model using MTT method, and cell profile with flow cytometry method. The results of cytotoxic test showed that 19 extracts had cytotoxic activity with IC50 100-300 μg/ml. One plant showed strong activity i.e. Gerrardanthus macrorhizus Harv. ex Benth. & Hook.f. tuber with IC50 value 73.5 μg/mL. Root tuber extract did not affect the MCF-7 cell cycle profile but increased cell population in sub G1 indicating apoptosis induction, potentially developed as chemopreventive agent.

 

Abstrak

Indonesia kaya akan berbagai jenis tumbuhan yang potensial sebagai bahan riset berbasis kearifan lokal dalam penemuan senyawa baru berkhasiat obat. Kekayaan tersebut terhimpun dalam hasil penelitian riset tumbuhan dan jamu (Ristoja) tahun 2012. Penelitian ini merupakan analisis lanjut dari Ristoja 2012 yang bertujuan untuk menguji aktivitas sitotoksik 20 bagian tumbuhan yang dimanfaatkan untuk pengobatan tradisional kanker di Indonesia. Ekstraksi dilakukan dengan metode infusa menggunakan pelarut air kemudian dikeringkan dalam oven suhu 40o C hingga diperoleh ekstrak kering. Uji aktivitas sitotoksik dilakukan pada model sel kanker payudara MCF-7 menggunakan metode MTT, dan profil siklus sel dengan metode flow cytometry. Hasil uji sitotoksik menunjukkan, 19 ekstrak memiliki aktivitas sitotoksik dengan IC50 100-300 μg/mL. Satu tumbuhan menunjukkan aktivitas kuat yaitu umbi akar batu (Gerrardanthus macrorhizus Harv. ex Benth. & Hook.f.) dengan nilai IC50 73,5 μg/mL. Ekstrak umbi akar batu tidak mempengaruhi profil siklus sel MCF-7 namun meningkatkan populasi sel di sub G1 yang mengindikasikan terjadinya apoptosis, sehingga potensial dikembangkan sebagai agen kemopreventif.


Keywords


tumbuhan obat; Gerrardanthus macrorhizus; sitotoksik; MCF-7; pengobat tradisional

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