Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats

Eva Rachmi, Yudanti Riastiti

Fagraea racemosa leaf extract inhibits oxidative stress-induced liver damage in Wistar rats

Abstract


Latar belakang: Kemampuan hati mengatasi stres oksidatif dapat ditingkatkan dengan konsumsi antioksidan eksogen yang berasal dari alam. Penelitian ini ditujukan untuk mempelajari kemampuan hepatoprotektif dari ekstrak metanol  daun  Fagraea racemosa, dengan menggunakan CCL4 sebagai model sumber radikal bebas.

Metode: Tiga kelompok perlakuan tikus Wistar  (enam ekor per  kelompok), masing-masing diberi dosis ekstrak berturut-turut 50, 100, 200 mg/kg bb per oral, sekali perhari selama 30 hari. CCl4 diinjeksikan intraperitoneal kepada ketiga kelompok , dua kali per minggu (1,5 ml/kg bb).  Sebagai pembanding, digunakan dua kelompok kontrol, yaitu kontrol normal dan kontrol CCl4.  Pada hari ke-30, tikus dibunuh dan hati diwarnai dengan hematoksilin-eosin. Perubahan histopatologi ditentukan berdasar derajat steatosis, degenerasi hidropik, dan inflamasi. Data dianalisis dengan Anova dan uji post hoc LSD (p≤0.05) menggunakan SPSS versi 13.0

Hasil: Hasil menunjukkan perbaikan derajat degenerasi hidropik dan inflamasi (p≤0,05) pada ketiga kelompok perlakuan bila dibanding dengan kelompok kontrol CCl4. Tetapi, derajat steatosis meningkat pada kelompok perlakuan dosis  50 dan 100 mg/kg bb, dan kemudian menurun secara bermakna pada perlakuan 200 mg/kg bb.

Kesimpulan : Ekstrak methanol daun Fagraea racemosa  mampu melindungi hati dari radikal bebas yang dihasilkan dari CCl4. Hasil ini mengindikasikan bahwa Fagraea racemosa menjanjikan untuk dikembangkan sebagai suplemen antioksidan. (Health Science Indones 2011;2:46-51)

 

Abstract

Background: The ability of the liver in dealing with oxidative stress can be enhanced by consumption of exogenous antioxidants derived from nature. This study aimed to explore the hepatoprotective ability of Fagraea racemosa leaves methanolic extract against CCl4 exposure as a model of free radicals source.

Methods: Three different doses (50, 100, 200 mg/kg bw) were administered orally to three treatment groups of Wistar rats (six Wistar rats each), respectively, once per day for 30 days. CCl4 injected intraperitoneally to those three groups, twice a week (1,5 ml/kg bw).  Two control groups were provided that were one normal control group and one CCl4 control group.  On the 30th day, the rats were killed and its liver examined with Haematoxyllin eosin staining. Histopathological changes were graded based on the degree of steatosis, hydropic degeneration, and inflammation. Data were analyzed with ANOVA and LSD post hoc (p≤0.05) using SPSS version 13.0

Results: The results showed improvement between the three treatment groups and the CCl4 control group about the degree of hydropic degeneration and inflammation (P ≤ 0.05). However, there were significant increased of steatosis 50 and 100 mg/kg bw treatment groups, before its significantly decrease at 200 mg/kg bw treatment group (2.5 g/kg).

Conclusions: Fagraea racemosa leaves methanolic extract could protect liver from free radicals generated by CCl4. The result indicated that Fagraea racemosa has promising quality to be explored as antioxidant supplement. (Health Science Indones 2011;2:46-51)


Keywords


methanolic extract, Fagraea racemosa, hepatoprotective

Full Text: PDF

Refbacks

  • There are currently no refbacks.


---

Health Science Journal of Indonesia

p-ISSN: 2087-7021
e-EISSN: 2338-3437

Published by : Badan Litbang Kesehatan, Kementerian Kesehatan RI

Visitor of HSJI: View HSJI Stats


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

Health Science Journal of Indonesia has been indexed by :