Gambaran Peningkatan Kadar Inter Leukin-10 (IL-10) dan Tumor Necrosis Faktor – Alfa (TNF-α) dengan Gejala Klinis pada Penderita Malaria

Fridolina Mau, S.Si, M,Sc, Mefi Mariana Tallan

Abstract


Abstract

Malaria parasites develop in erythrocytes and naturally-acquired immune responses can result in either the elimination of the parasites or a persistent response. The cytokines are responsible for all the symptoms, pathological alterations and the outcome of the infection depending on the reciprocal regulation of the pro inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines. The aim of this study was to describe the level of IL-10 and TNF- α on malaria infection, using an analytic laboratory cross-sectional design. The serum levels of the cytokines TNF- α and IL-10 from 50 patients were evaluated by indirect ELISA. The results revealed that increased levels of IL-10 and TNF-α among respondents without clinical symptoms of malaria were higher compared to respondents with clinical symptoms of P. falciparum and P. vivax. Statistically, there was no significant association between clinical symptoms with increased cytokine IL-10 and TNF-α.The ratio of TNF-α / IL-10 in respondents with clinical symptoms and without clinical symptoms indicated that the respondent without clinical symptoms was higher than that of clinical symptoms. The study concluded that molecular basis of immune response of patients in the study site is still very good because of reciprocal response between pro-inflammatory cytokines and anti-inflammatory.

Key words : clinical symptom, cytokine TNF-α, IL-10, malaria

Abstrak

Ketika parasit berkembang di dalam sel darah merah, respon kekebalan tubuh yang secara alami diperoleh dapat mengakibatkan penghapusan/pembersihan parasit atau respon persisten dimediasi oleh sitokin yang mengarah ke immunopatologi. Sitokin yang ikut bertanggung jawab untuk semua gejala, perubahan patologi yang dihasilkan tergantung pada hubungan timbal balik antara sitokin pro inflamsi (TNF-α) dan anti inflamasi (IL-10). Tujuan penelitian adalah menggambarkan peningkatan kadar IL-10 dan TNF-α pada malaria. Metode penelitian ini menggunakan rancangan analitik laboratorium secara potong lintang. Kadar TNF-α dan IL-10 diukur dengan menggunakan metode ELISA. Hasil; peningkatan kadar IL-10 dan kadar TNF-α pada responden malaria tanpa gejala klinis lebih tinggi dibandingkan dengan responden dengan gejala klinis P. falciparum maupun P.vivax. Secara statistik tidak ada hubungan signifikan antara gejala klinis dengan peningkatan sitokin IL-10 dan TNF-α. Rasio TNF-α/IL-10 pada responden dengan gejala klinis dan tanpa gejala klinis menunjukkan pada responden tanpa gejala klinis lebih tinggi dibandingkan dengan gejala klinis. Kesimpulan : Secara molekuler respon immun penderita di lokasi penelitian masih sangat baik dimana masih ada respon timbal balik antara sitokin pro-inflamasi dan anti- inflamasi.

Kata kunci : gejala klinis, malaria, sitokin TNF-α, IL-10


Keywords


clinical symptom, cytokine TNF-α, IL-10, malaria

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